Cathepsin B (Protein name
), CATB_PIG from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Gene name:
CTSB;
Protein name:
Cathepsin B;
Alternative:
Organism:
Pig (Sus scrofa).
General Annotation
Sub Unit:
Interacts with SRPX2. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond.
Function:
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
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12% SDS-PAGE Analysis
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤ 5.2%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤ 8.3%
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Recovery
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Recovery was determined by spiking various levels of ACE into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
92-105
Plasma
96
93-104
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1：2
1:4
1:8
1:16
serum(n=5)
88-101%
90-103%
93-105%
92-102%
EDTA plasma(n=5)
89-99%
93-101%
89-98%
90-105%
heparin plasma(n=5)
92-101%
94-105%
98-107%
95-108%
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References
1.
"Molecular cloning and polymorphism of cathepsin B (CTSB) gene in porcine." Chen L.
,
Li X.W.
,
Zhu L.
,
Li Q.
,
Li M.Z.
Submitted (2006-11) to the EMBL/GenBank/DDBJ databases
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Cited for: NUCLEOTIDE SEQUENCE [MRNA]
Vaild Sequence:
